Studies on in vitro Growth Rate of Culturable Gut Bacterial Flora of Three Stem Borers Infesting Rice (Oryza sativa)


  • Subrata Goswami Dept. of Entomology and Agricultural Zoology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh (221 005), India & Dept. of Entomology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh (482 004), India & Crop Protection Division, ICAR-National Rice Research Institute, Cuttack, Odisha (753 006), India
  • S.B. Das Dept. of Entomology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh (482 004), India
  • P.C. Rath Crop Protection Division, ICAR-National Rice Research Institute, Cuttack, Odisha (753 006), India



Growth rate, Gut bacteria, Host insect, Oryza sativa, Rice, Stem borers


Stem borers are the most devastating pest of rice globally. The present experiment was conducted to compare the in vitro growth rate under different temperature conditions of culturable gut bacteria isolated from three different rice stem borers viz., Scirpophaga incertulas (YSB), Chilo suppresalis (SSB) and Sesamia inferens (PSB). Growth was recorded spectrophotometrically by measuring optical density at 660 nm. The gut bacterial consortia of YSB and PSB grew better than those of SSB at lower temperatures (15 and 20 °C) while that of PSB performed equally well in the entire range of temperatures. The gut bacterial consortium of SSB grew luxuriantly with the increase in temperature with the peak growth at 30 °C. There was no marked difference in the growth trend of all the bacterial isolates at 30 °C. These results indicate differential temperature preferendum of gut flora and give insights into their possible relation with behavioural response of the host insect species at different temperature.


Download data is not yet available.


Ane, N.U., Hussain, M., 2016. Diversity of insect pests in major rice growing areas of the world. Journal of Entomology and Zoology Studies 4(1), 36-41.

Cantindig, J.L.A., Hoeng, H.L., 2003. Rice Doctor. International Rice Research Institute, Manila, Philippines. pp. 1-10.

de Almeida, L.G., de Moraes, L.A., Trigo, J.R., Omoto, C., Consoli, F.L., 2017. The gut microbiota of insecticide-resistant insects houses insecticide-degrading bacteria: A potential source for biotechnological exploitation. PLOS One 12(3), 174754-174773. DOI:

Dhakal, A., Poudel, S., 2020. Integrated pest management (IPM) and its application in rice - A review. Reviews in Food and Agriculture 1(2), 54-58. DOI:

Dhaliwal, G.S., Arora, R., 2009. Integrated Pest Management. Kalyani Publishers, New Delhi, India. p. 369.

Douglas, A.E., 2015. Multiorganismal insects: Diversity and function of resident microorganisms. Annual Review of Entomology 60(1), 17-34. DOI:

Engel, P., Moran, N.A., 2013. The gut microbiota of insects - diversity in structure and function. Federation of European Microbiological Societies Microbiology Reviews 37(5), 699-735. DOI:

Feldhaar, H., 2011. Bacterial symbionts as mediators of ecologically important traits of insect hosts. Ecological Entomology 36(5), 533-543. DOI:

Hosokawa, T., Fukatsu, T., 2020. Relevance of microbial symbiosis to insect behavior. Current Opinion in Insect Science 39, 91-100. DOI:

Itoh, H., Hori, T., Sato, Y., Nagayama, A., Tago, K., Hayatsu, M., Kikuchi, Y., 2018. Infection dynamics of insecticide-degrading symbionts from soil to insects in response to insecticide spraying. The ISME Journal 12, 909-920. DOI:

Jang, S., Kikuchi, Y., 2020. Impact of the insect gut microbiota on ecology, evolution, and industry. Current Opinion in Insect Science 41, 33-39. DOI:

Jing, T.Z., Qi, F.H., Wang, Z.Y., 2020. Most dominant roles of insect gut bacteria: Digestion, detoxification, or essential nutrient provision? Microbiome 8, 38. DOI:

Krishnaiah, K., Varma, N.R.G., 2018. Changing insect pest scenario in the rice ecosystem - A national perspective. In: Rice Knowledge Management Portal (RKMP). Directorate of Rice Research, Rajendranagar, Hyderabad (500 030), India. pp. 1-28.

Li, Q., Sun, J.X., Qin, Y.G., Fan, J., Zhang, Y., Tan, X.L., Hou, M.L., Chen, J.L., 2020. Reduced insecticide sensitivity of the wheat aphid Sitobion miscanthi after infection by the secondary bacterial symbiont Hamiltonella defensa. Pest Management Science 77(4), 1936-1944. DOI:

Liberti, J., Engel, P., 2020. The gut microbiota - brain axis of insects. Current Opinion in Insect Science 39, 6-13. DOI:

McLean, A.H.C., Parker, B.J., Hrček, J., Henry, L.M., Godfray, H.C.J., 2016. Insect symbionts in food webs. Philosophical Transactions of the Royal Society B: Biological Sciences 371(1702), 20150325. DOI:

Orozco-Flores, A.A., Valadez-Lira, J.A., Oppert, B., Gomez-Flores, R., Tamez-Guerra, R., Rodriguez-Padilla, C., Tamez-Guerra, P., 2017. Regulation by gut bacteria of immune response, Bacillus thuringiensis susceptibility and hemolin expression in Plodia interpunctella. Journal of Insect Physiology 98, 275-283. DOI:

Pathak, H., Nayak, A.K., Jena, M., Singh, O.N., Samal, P., Sharma, S.G., 2018. Rice research for enhancing productivity, profitability and climate resilience. ICAR-National Rice Research Institute, Cuttack (753 006), Odisha, India. p. 542.

Prasad, R., Shivay, Y.S., Kumar, D., 2017. Current status, challenges, and opportunities in rice production. In: Rice Production Worldwide. (Eds.) Chauhan, B.S., Jabran, K. and Mahajan, G. Springer, Cham, New York. pp. 1-32. DOI:

Ramya, S.L., Venkatesan, T., Murthy, K.S., Jalali, S.K., Varghese, A., 2016. Degradation of acephate by Enterobacter asburiae, Bacillus cereus and Pantoea agglomerans isolated from diamondback moth Plutella xylostella (L), a pest of cruciferous crops. Journal of Environmental Biology 37(4), 611-618.

Shukla, S.P., Plata, C., Reichelt, M., Steiger, S., Heckel, D.G., Kaltenpoth, M., Vilcinskas, A., Vogel, H., 2018. Microbiome-assisted carrion preservation aids larval development in a burying beetle. Proceedings of the National Academy of Sciences 115(44), 11274-11279. DOI:

Wong, A.C.N., Holmes, A., Ponton, F., Lihoreau, M., Wilson, K., Raubenheimer, D., Simpson, S.J., 2015. Behavioral microbiomics: A multi-dimensional approach to microbial influence on behavior. Frontiers in Microbiology 6, 01359. DOI:




How to Cite

Goswami, S. et al. 2023. Studies on in vitro Growth Rate of Culturable Gut Bacterial Flora of Three Stem Borers Infesting Rice (Oryza sativa). Research Biotica. 5, 3 (Jul. 2023), 97–101. DOI:



Research Articles